hTERT在肾癌中的表达及临床意义

Expression of human telomerase reverse transcriptase in renal cell carcinoma and its clinical significance

目的检测人端粒酶逆转录酶(hTERT)在肾癌中的表达及其临床意义。方法采用RT-PCR及Western blot方法检测45例肾癌组织、45例配对的癌旁肾组织及786-0细胞系中hTERT mRNA及蛋白的表达情况,观察其表达与肿瘤大小、临床分期、病理组织分型、分级之间的关系。结果hTERT mRNA及蛋白在肾癌组织中呈高表达(P=0.000),其表达与肾癌的肿瘤大小、临床分期、病理组织分型、分级之间无关(P>0.05)。结论肾癌组织中hTERT表达较高,可尝试用于肾癌的诊断和预测,并为基因治疗提供了思路。

Objective To investigate the expression of human telomerase reverse transcriptase （hTERT） in renal cell carcinoma （RCC） and its clinical significance. Methods The expression levels of hTERT mRNA and protein were detected using RT-RCR and Western blotting in 45 RCC tissues, 45 adjacent tissues and 786-0 cell line, and the associations of hTERT expression with the tumor size, clinical stage, pathological type and grade were evaluated. Results hTERT mRNA and protein was expressed at significantly higher levels in RCC tissues than in the adjacent tissues （P=0.000）, and no correlation ofhTERT expression was found with the tumor size, clinical stage, pathological type or grade. Conclusion hTERT might serve as a diagnostic and prognostic marker for RCC, and also shed light on the new clues for gene therapy of RCC.