二妙丸系列类方有效部位群药效学比较研究

Comparative study for Pharmacodynamics on Effective Parts of Ermiaowan Serial Prescriptions

目的:比较二妙丸系列类方(二妙丸、三妙丸、四妙丸及加味四妙丸)有效部位群抗湿热下注证及痛风药效的关联性。方法:采用醋酸刺激引起小鼠扭体实验、二甲苯致小鼠耳肿胀实验、小鼠高尿酸模型的降尿酸实验及小鼠碳粒廓清实验,评价二妙丸系列类方药效作用。结果:各系列方均具有显著的免疫增强作用,多糖为免疫增强有效部位;各系列方有效部位群均具有显著的抗炎作用,镇痛、降尿酸作用依次增强,其中四妙丸及加味四妙丸有效部位群镇痛、降尿酸作用更强。结论:二妙丸系统类方对湿热下注证及抗痛风作用具有共同的药效物质基础,从基本方到系列类方,药效物质基础及效应的变化存在着一定的联系和作用规律。

Objective： To compare the relation of effective parts of Ermiaowan（EMW） serial prescriptions （Ermiaowan, Sanmi- aowan, Simiaowan and modified Simiaowan） which can cure downward flow of damp - heat syndrome and gout. Methods： these experi- ments, mouse acetic - acid - induce twisting experiment, mouse xylene - induced auricular swelling experiment, an animal model of hyperuricemia to decreasing serum uric acid and carbon particle clearance test, were carried out to evaluate the pharmacodynamic efects of EMW serial prescriptions. Results： All effective parts of EMW serial prescriptions had obvious effect to reisit inflamaton and increase immune function; the effects to relieve pain and decrease uric acid had been reinforced gradually, but effective parts of Simiaowan and modified Simiaowan of that had stronger power. Conclusion： EMW serial prescriptions had same pharmacodynamic substance foudation on downward flow of damp - heat syndrome and anti - gout. Pharmacodynamic substance - foundation and effective change existed cer- tain relation and regularity.