冠心病患者载脂蛋白E基因多态性及其与血脂的关系

Relation between apoliprotein E (apoE) gene polymorphisms in patients with coronary heart disease (CHD) and serum lipids

目的通过研究冠心病患者apoE基因多态性及其与血脂的关系,探讨冠心病的遗传易感因素。方法选取80例冠心病患者,60例健康人,通过聚合酶链反应-限制性片段长度多态性测定apoE基因多态性。结果CHD组携带E3/4基因型较对照组增高,对照组携带E3/3基因型较CHD组增高(P<0.05)。E4等位基因频率分布CHD组明显高于对照组(P<0.05)。CHD组中TG、LDL-C血浓度较对照组增高,E3/4和E3/3基因型较E2/3基因型血中TG、LDL-C增高更明显(P<0.05)。结论apoEE4等位基因可能是冠心病的遗传易感因子,不同的基因型可能通过影响血脂而与冠心病发病有关。

[Objective] The study aim is to explore the hereditary susceptibility of people to develop CHD through studying the relationship between apoE gene polymorphisms in patients with CHD and serum lipids. [Methotis] 80 people with CHD were chosen as study group, 60 normal people were chosen as the control group. Polymerase chain reaction-restrlction fragment length polymorphism （PCR-RFLP） was used to detect the apoE gene polymorphisms. [Results] The frequencies of E3/4 genotypes in CHD group were significantly higher than those in control group. The frequencies of E3/3 were lower in CHD group than those in control group. A significant difference was found in E4 allele between two groups （P〈0.05）. The serum levels of TG and LDL-C in CHD group were significantly higher than those in control group （P 〈0.05）. The serum levels of TG and LDL-C in E3/4 genotypes and E3/3 genotypes were significantly higher than those in E2/3 genotypes （P 〈0.05） in CHD group. [Conclusion] E4 allele is probably the genetic marker of CHD. Apo-e gene affects the CHD maybe through influence of the CHD patients, serum lipids.