奥曲肽联合5-FU对体外培养的大肠癌细胞增殖及突变型p53蛋白表达的影响

The effects of combination of Octreotide and 5-FU on the proliferation and expression of mutant p53 protein in human colorectal cancer cells in vitro

目的:通过生长抑素类似物奥曲肽(OCT)联合5-FU对体外培养的大肠癌细胞作用及对p53蛋白的影响为大肠癌的临床治疗提供一定的理论依据。方法:采用结肠癌细胞原代培养,采用MTT比色分析法测定奥曲肽与5-FU联合是否抑制作用增强;流式细胞仪间接免疫荧光技术检测药物作用后的肿瘤细胞突变型p53蛋白表达情况。结果:5-FU和奥曲肽联合应用对大肠癌的抑制率高于单独应用。5-FU和1.0μg/mlOCT组均抑制突变型p53蛋白的表达,联合用药组更为明显。结论:奥曲肽和5-FU都能抑制肿瘤细胞的突变型p53蛋白的表达,且联合应用抑制作用强于两者的单独应用,这可能是奥曲肽能增强5-FU对结肠癌细胞抑制作用的机制之一。

Objective To provide a few certain proofs for clinieal therapy with the effects of combination of Octreotide （OCT） and 5 - FU on the proliferation and expression of mutant p53 protein in human colorectal cancer cells in vitro. Method The efficaey of OCT combined with 5 - FU against the cancer cells was observed by MTT assay. Indirect immune fluorescent labeling and flow cytometry was employed to investigate the exprossion of mutation p53 protein. Results Combination of OCT and 5 - FU enhanced the action, and the inhibition ratio was higher than 5 - FU or OCT alone. Row eytometric analysis demonstrated a decreased FI, the expression of mutant p53 protein was decreased. Conclusion Both OCT and 5 - FU can inhibite the expression of mutant p53 protein in the cancer ceils, and the inhibition efficacy is higher than 5 - FU or OCT alone in vitro,so this may be one of the mechanisms that OCT can enhance the inhibition ratio of 5 - FU on colorectal cancer cells.