pCD-Em10对泡球蚴感染小鼠免疫应答的影响

Effects of pCD-Em10 on immune response in mice infected with alveolar hydatid cyst of Echinococcus multilocularis

目的:探讨pCD-Em10免疫对泡球蚴感染小鼠免疫应答的影响。方法:将pCD-Em10肌肉注射免疫BALB/c鼠,免疫后8周用Em原头节进行攻击感染,感染后18周剖杀小鼠,计算减蚴率;取脾并分离脾细胞,流式细胞仪检测脾CD4+和CD8+T淋巴细胞亚群的百分比;用EmAg或ConA刺激培养脾细胞,收集脾细胞培养上清液,用试剂盒检测脾细胞培养上清液的IL-2、IFN-γ、TNF-α和IL-4水平;流式细胞仪检测脾细胞的凋亡发生率,同时设有BCG和PBS对照。结果:pCD-Em10免疫组的减蚴率为71.17%,脾CD4+T细胞亚群显著增加,IFN-γ和TNF-α水平升高,IL-4水平降低,脾细胞凋亡发生率明显低于PBS对照组。结论:pCD-Em10可诱导泡球蚴感染小鼠产生一个保护性的免疫反应。

Objective：To investigate the influence of pCD-Em10 on immune responses in mice infected with alveolar hydatid cyst of Echinococcus multilocularis（Em）.Methods：BALB/c mice were intramuscularly vaccinated with pCD-Em10 and then challenged with Em protoscoleces at the 8 w after vaccination.The mice were killed in 18th week of infection to count the rate of reduced alveolar hydatid cyst weight;The spleens were gathered to separate splenocytes,and the subsets of CD4^＋and CD8^＋T cells were measured by FACsort.The splenocytes were cultured by stimulation with EmAg or ConA,their supernatants were gathered to measure level of IL-2,IFN-γ,TNF-α and IL-4 by ELISA Kits.Apoptotic rate of splenocytes was measured by FACsort.BCG or PBS injection served as control.Results：In the group of PCD-Em10 immunization,the rate of reduced alveolar hydatid cyst weight was 71.17%,CD4^＋ subset increased obviously,and the levels of IFN-γ and TNF-α increased remarkably,but that of IL-4 decreased.Apoptotic rate in the pCD-Em10 group was lower than that in the PBS control.Conclusion：pCD-Em10 may induce a protective immune response in mice infected with alveolar hydatid cyst of Echinococcus multilocularis.