纤维素酶高产菌株的诱变选育

Screening of cellulase high-yield strains by mutant

以绿色木霉13010为出发菌株,利用诱变因子的协同作用,对其进行了诱变育种,得到酶活力较高的突变菌株绿色木霉GL13010。实验结果表明:紫外线、亚硝酸钠诱变因子对绿色木霉13010菌体细胞的致死作用表现出相似的趋势,在一定范围内都与诱变剂量呈线性正相关。在协同诱变条件下,多轮反复诱变绿色木霉13010后,菌株的酶活力有大幅提高,最终筛选得到了产纤维素酶活力单位提高了70.63%的菌株-GL13010。

In this research, a strain capable of producing cellulase-Trichoderma viride 13010 was applied, T. viride 13010 was mutated by synergistic mutation for increasing cellulase production. We got a high cellulase product strain named T. viride GL13010. The main results were as follows： Two mutation factors （UV, sodium nitrite） possessed mortal ratio trend, which positively related to the mutation factors＇ concentration. The cellulase production of mutation strain, named T. viride GL13010 could increase70.63% after repeated Synergistic mutation. It illuminated that complex mutation could weaken repellence and saturation by single mutation factor.