雄激素受体基因第一外显子微卫星多态性与多囊卵巢综合征的相关性研究

Association between androgen receptor gene CAG trinucleotide repeat polymorphism and polycystic ovary syndrome

目的:研究雄激素受体(AR)基因第一外显子微卫星多态性与多囊卵巢综合征(PCOS)发病的相关性。方法:采用聚合酶链反应(PCR)及变性聚丙烯酰胺凝胶电泳分离DNA片段的方法,检测了64例PCOS患者和58例正常对照者的AR基因第一外显子微卫星多态性。结果:1共有28个AR-CAG等位基因,其范围在PCOS组是11～36(CAG)s,在对照组是12～31(CAG)s;2在PCOS组,长等位基因、短等位基因及双等位基因的(CAG)s平均数与对照组相比,差异均无统计学意义。结论:AR基因第一外显子微卫星多态性与PCOS的发病似无直接关系。

Objective：To study the association between CAG trinucleotide repeat polymorphism in exon 1 of androgen receptor （AR） gene and polycystic ovary syndrome （PCOS）. Methods： Frequency distributions of CAG repeat alleles of AR gene of 64 PCOS patients and 58 controls were analyzed by PCR and denaturing acrylamide gels electrophoresis. Results：Twenty AR-CAG alleles in our population were found. The CAG repeats ranged from 11-36 in patients and 12-31 in controls. There were no differences in the mean CAG repeat number between patients and controls when both alleles were eonsiogether or separately （all P ：〉 0.05）. Conclusion. There is no association between AR-CAG trinucleotide repeat microsatellite polymorphism and PCOS.