甘蓝型油菜芥酸含量的遗传与QTL定位

Inheritance and QTL Identification of Erucic Acid Content in Rapeseed(Brassica napus L.)

以低芥酸油菜品系APL01与高芥酸品种M083杂交获得的6个基本世代(P1、P2、F1、B1、B2和F2)为材料,利用主基因+多基因混合遗传模型对油菜脂肪酸组成中的芥酸含量进行遗传分析。结果表明:芥酸含量由2对加性-显性主基因控制,2对主基因的加性效应值分别为-12.27和-8.83,显性效应值分别为0.35和1.69,加性效应显著大于显性效应;芥酸含量的主基因遗传率较高,为92.54%~96.72%。以(APL01×M083)BC1F1为作图群体,利用251个分子标记,构建了由19个连锁群组成的分子标记遗传图谱,并利用W inQTLCart 2.0对种子芥酸含量进行QTL扫描,获得2个与芥酸含量相关的QTL,其中qER8位于N8连锁群的m11 e37b^A0226Ba267区间,效应值为-8.32,可解释芥酸含量表型变异为16.74%;qER13位于N13连锁群的A0301Bb398~m18 e46区间,效应值为-9.12,可解释芥酸含量表型变异为31.32%。qER8和qER13两侧分子标记m11 e37b、A0226Ba267、A0301Bb398和m18 e46可用于芥酸含量的标记辅助选择。

A low erucic acid content line APL01 （Brassica napus L. ） was crossed with a high erucic acid content variety M083（B. napus L. ）, and six basic generations（P1 ,P2 ,F1 ,B1 ,B2 and F2） derived from this cross were used to analyze the genetic model by applying major gene and polygene mixed model. Results showed that erucic acid content in this cross was controlled by two pairs of additive-dominance major genes. The additive effects of the two pairs of genes, which were - 12. 27 and -8. 83, respectively, were larger than the dominance effects, which were 0. 35 and 1.69 respectively. The heritabilities of major genes were 92. 54% - 96. 72%. A genetic recombination map of Brassica napus L. was constructed based on the segregation population of （ APL01 × M083） BC1 F1 and 251 molecular markers, which contained 19 linkage groups. Two QTLs, qER8 and qER13, related to erucic acid content were identified from this cross by WinQtlCart 2.0. qER8 was located in the region of m11e37b - A0226Ba267 on linkage group N8, which could explain 16. 74% of the erucic acid content variation in the population, qER13 was located in the region of A0301Bb398 - m18e46 on N13, and accounted for 31.32% of phenotypic variation. The effects of the two QTLs were larger, and the molecular markers flanking the two QTLs could be used for marker assisted selection in low erucic acid content rapeseed breeding.