非阻塞性输精管滤过装置节育术的精浆中性α-糖苷酶的检测

Studies on changes in plasma membrane fluidity during epididymal sperm maturation of rat

本文采用ＷＨＯ推荐的改良精浆中性α－糖苷酶的测定方法测定输精管滤过装置节育术（ＩＶＤ组）和输精管结扎术（结扎组）的精浆中性α－糖苷酶活性。术前两组精浆均测出中性α－糖苷酶活性：ＩＶＤ组为４３．５０±２９．０１ｍＵ／每次射精（ｘ±ｓ）；结扎组为４７．８１±３１．２０（ｘ±ｓ）ｍＵ／每次射精；两组间无显著差异（Ｐ＞０．０５）。术后６、１２个月ＩＶＤ组分别有９１．５７％和７９．１７％的精浆测出中性α－糖苷酶活性；而结扎组仅有４．４８％和４．２４％的精浆测出中性α－糖苷酶活性；两组间均有非常显著的差异（Ｐ＜０．００１）。结果提示：ＩＶＤ组术后一年内其大部分受试对象的精浆中仍有附睾液存在。

During epididymal sperm maturation, the sperm structure and function were modified. The changes in plasma membrane fluidity of spermatozoa from epididymal caput, corpus and cauda of adult SD rats were studied with spin label, 5-Doxyl-stearic acid (5-DSA) and 16-Doxyl-stearic acid (16-DSA), and ESR spectroscopy technique. The hypoosmotic swelling (HOS) test was designed to measure the ability of the sperm plasma membrane to respond osmotically. The results showed that the fluidity of sperm membrane lipid declined gradually from caput to cauda. There was obvious difference between sperm in corpus and in cauda ( P <0.01). The ability of sperm membrane to respond osmotically also decreased, especially from caput to corpus ( P <0.01). The lipid peroxidation reaction during sperm maturation may be one of the causes declining the membrane lipid fuidity.