安宫牛黄丸及重金属组分对内毒素脑损伤大鼠脑电图的影响

Effect of Angong Niuhuang Pill and the heavy metal constituents on EcoG of brain damage in rats caused by LPS

目的:评估朱砂雄黄在安宫牛黄丸"醒脑开窍"中所发挥的作用,为安宫牛黄丸组方的合理性提供实验依据。方法:采用药物定量脑电图的方法,以内毒素尾静脉注射复制内毒素脑损伤模型,观察含与不含朱砂雄黄的安宫牛黄丸及朱砂、雄黄对内毒素致脑损伤脑电图δ波、β波功率及相对功率的影响。结果:内毒素可使大鼠脑电图的慢波(δ波)功率和相对功率增加,而快波(β波)功率和相对功率降低。几种药物均可降低内毒素(LPS)脑损伤大鼠脑电δ波功率和相对功率;全方、朱砂雄黄、单雄黄可提高β波的功率和相对功率,朱砂可提高β波相对功率,简方的作用不明显。结论:全方对内毒素脑损伤脑电图有明显的脑电激活作用,朱砂雄黄及单独朱砂、雄黄和全方的作用相似,推测朱砂雄黄可能是安宫牛黄丸醒脑开窍作用的重要物质基础之一。

AIM： To evaluate the actions of cinnabaris and realgar in arousing brain and opening the orifices of Angong Niuhuang Pill, and to provide experimental basis for the rationality of Angong Niuhuang Pill buildup. METHODS： The brain damage model was made by LPS injection through caudal vein. With quantitative electroencephalogram of medicine methods, effects of Angong Niuhuang Pill, Angong Niuhuang Pill without cinnabaris and realgar, cinnabaris, realgar on the power and relative power of delta and beta waves of electrocorticogram （ECoG） were observed in the brain damage rats caused by LPS. RESULTS： LPS could increase the power and the relative power of the delta waves（0.5-3 Hz）, and reduce the power and the relative power of the beta waves （ 14-30 Hz）. All medicines could reduce the power and the relative power of the delta; Angong Niuhuang Pill, cinnabaris and realgar, realgar could increase the power and the relative power of the beta, cinnabaris could increase the relative power of the beta （ 14-30 Hz）, and Angong Niuhuang Pill without cinnabaris and realgar could not. CONCLUSION： Angong Niuhuang Pill has obvious activation on ECoG, and same as cinnabaris and realgar. The results of the present work allow us to put forward the hypothesis that cinnabaris and realgar are most likely one of the important material basis in arousing brain and opening the orifices of Angong Niuhuang Pill.