一株中度嗜盐菌的分离鉴定及其强化高盐有机废水处理的研究

Isolation and Identification of a Moderately Halophilic Bacterium and Its Bioaugmention in Intensifying High-salty Organic Wastewater Treatment

为探索中度嗜盐菌在高盐有机工业废水处理中的应用,解决高盐有机工业废水处理这一难题,从山东省威海市路道口盐场晒盐池盐水中分离一株嗜盐菌株YS-1,通过对该菌株进行原子力显微镜观察、生理生化测定,全细胞脂肪酸分析、16S rDNA序列同源性分析发现YS-1菌株16S rDNA序列与Halomonas sp.(AB167061)的亲缘关系最为接近,结合上述其他各项结果确定该菌株为盐单胞菌属(Halomonas sp.),属中度嗜盐菌;在SBR反应器中对该菌株进行强化高盐有机废水处理试验,结果表明,含盐12%,CODCr=1 494mg/L的高盐模拟有机废水,经72 h CODCr去除率为90.0%,120 h的CODCr去除率为98.1%,该菌株具有强化高盐有机废水处理的功能,通过分离筛选嗜盐菌强化高盐有机工业废水处理具有可行性.

In order to study the application of moderately halophilic bacterium in the hypersaline organic wastewater treatment, a moderately halophilic bacterium YS-1 was isolated from the salty water of a Ludaokou salina of Weihai in China. The polyphasic taxonomy study was conducted by identifying its morphological features by AFM by studying the features of culture, measuring the physiologic-biochemical index and analyzing 16 S rDNA sequence homology. Phylogenetic analysis based on 16 S rDNA gene sequences showed that the closest relative of the strain was Halomonas sp. （AB167061） Experiments of intensifying hypersaline organic wastewater treatmented processing in the SBR demonstrate a significant result. The wastewater salt content of initial wastewater was 12 % and the initial CODcr was 1 494 mg/L. After 72h＇s, the removal efficiency of CODcr achieved 90.0 %, and after 120 h, it achieved 98.1%. The experiments also showed that the hypersaline organic wastewater treatment had the feasibility acclimating of halophilic bacteria and running of processing system.