针刺对肥胖大鼠Leptin-下丘脑NPY轴的影响

Effect of acupuncture on leptin-hypothalamic NPY chain in obese rat

目的:探讨针刺对肥胖大鼠血清瘦素(Leptin)水平、下丘脑神经肽Y(neuropeptide Y,NPY)等指标的影响,研究针刺减肥机理,为临床应用针刺减肥提供理论和实验依据。方法:选择1月龄SD雄性大鼠20只,采用自制高脂饲料制备肥胖大鼠模型,分为肥胖组(6只)与针刺组(6只)。应用免疫组化方法测定脂肪细胞瘦素表达,应用放射免疫分析方法测定血清Leptin及下丘脑NPY水平。结果:与肥胖组相比,针刺后脂肪组织瘦素表达减弱,血清瘦素水平降低,下丘脑NPY水平降低。结论:针刺对食源性肥胖大鼠的疗效显著,其减肥效果可能是通过Leptin-下丘脑NPY轴发挥作用。

Objectives： To investigate the effect of and the contents of hypothalamic neuropeptide Y acupuncture on weight, leers index, serum leptin level in obese rat fed by self-prescribed high calorie diet, research the mechanism of weight-lose function of acupuncture and provide a principle for practice of acupuncture in clinic. Methods： The obese rats were prepared by self-prescribed high calorie diet. Immunohistochemical analysis of leptin in celiac adipocytes were performed. The serum leptin and hypothalamic NPY were measured. Results： Comparing with obese rat without acupuncture, in acupuncture obese rat, immunohistochemical stainings of leptin were weaker, the level of serum leptin decreased, and the hypothalamic neuroprptide Y level decreased. It suggested that the weight lose effect of acupuncture on obese rat was marked, and the machanism responsible for this was the affect of acupuncture on leptin-hypothalamic neuropeptide Y chain. Conclusion： The effect of acupuncture on obese rat fed by self-prescribed high calorie diet was good, which might be obtained by its effect on leptin- hypothalamic neuropeptide Y chain.