高效液相色谱法测定双黄连粉针中黄芩甙和绿原酸的含量

Determination of the Contents of Baicaline and Chlorogenic Acid in Shuanghuanglian Powdery Injection by Method of HPLC

本文采用高效液相色谱法，测定中药制剂双黄连粉针中黄芩甙和绿原酸含量。流动相分别是0．1mol／L磷酸二氢钾：乙酸：四氢呋喃（100：10：19）和甲醇-10％磷酸缓冲液（10：90），流速分别是0．8和1ml／min；色谱柱ERC-ODS-1161（6×100mm），柱温55℃；黄芩甙以电化学为检测器，检测电压是＋650mv；绿原酸以紫外为检测器，检测波长274nm；结果：该方法的最小检测浓度分别为0．025mg／L和0．1mg／L，平均回收率分别为100．1％和100．4％，重现性好。本方法测定快速、简便、准确，适用于含黄芩甙和绿原酸的中药制剂的含量测定。

The contents of baicaline and chlorogenic acid in Shuanghuanglian powdery injection have been determined by themethod of HPLC under following conditions:column-ERC-ODS-1161,6 × 100mm;temperature 55℃;flow phase of baicaline-0.1 mol/LKHPO,acetic acid and tetrahydrofuran(1000：100:190);flow phase of Chlorogenic acid-methanol and 10%phosphate-buffer(10:90);flowrate 0.8ml/min;volt of ECD-detecor of baicalinee-＋650mv;wavelength of UV-detector of chlorogenic acid 274nm.The resultsshowed that the minimun determinable concentrations of baicaline and chlorogenic acid were 0.025mg/L and 0.1mg/Lrespectively,with an average recovery of 100.1% and 100.4% respectively.This method is highly reproducible,rapid,simple,convenient,accurate and suitable for the content determination of baicaline and chlorogenic acid in Chinese medicinal preparations.