摘要
目的:鉴定卡介菌多糖核酸诱导小鼠产生干扰素γ的能力。方法:分别采用生理盐水、卡介菌多糖核酸和脂多糖处理Balb/c小鼠,逆转录聚合酶链反应(RT-PCR)技术扩增编码小鼠干扰素γ(mIFNγ)cDNA,通过RT-PCR产物来评价卡介菌多糖核酸诱导mIFNγ表达的能力,并设β-actin为对照。结果:生理盐水组只扩增出β-actin条带,未扩增出mIFNγ cDNA,卡介菌多糖核酸组和脂多糖组的RT-PCR产物中均有mIFNγ和β-actin cDNA条带;与脂多糖组比较,卡介菌多糖核酸组的mIFNγ表达显著增强(P<0.01)。结论:卡介菌多糖核酸能有效诱导mIFNγ的表达。
OBJECTIVE： To detect the ability of polysaccharide nucleic acid fraction of bacillus calmette guerin（BCG- PSN） in inducing mIFNγ in mouse. METHODS： The Balb/c mice were treated with saline, BCG - PSN and lipopolysaccha- ride（LPS） respectively, and mIFNγ cDNA was amplified by RT PCR. The ability of BCG PSN in inducing mIFNγ in mouse was identified through RT - PCR product with β-actin as control. RESULTS： In normal sodium group, only β-actin but not the mIFNγ cDNA was detected, but in groups treated with BCG PSN or LPS, mIFNγ and β-actin cDNA were all detected in RT- PCR amplification products. As compared with LPS group, there was stronger expression of mIFNγ in BCG PSN group （P〈0.01）. CONCLUSION： BCG-PSN can effectively induce the expression of mIFNγ.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第7期506-507,共2页
China Pharmacy
