AGPase反义基因转化番茄研究

Study on Genetic Transformation of Tomato Using AGPase Anti-Sense Gene

将含有魔芋AGPase反义基因的质粒pBAGP通过冻融法转化到根癌农杆菌(Agrobacterium tumefaciens)菌株LBA4404中,再采用叶盘法将其转化进番茄(Lycopersicon esculentumMill.)栽培品种"合作908"中,获得含AGPase基因的番茄抗性植株。最后,经卡那抗性鉴定、NPTⅡ基因和AGPase基因PCR扩增和PCR-Southern杂交检测表明,反义AGPase基因成功整合到番茄基因组,为番茄改良品质育种奠定了材料基础。

Carrying an Amorphophallus adenosine diphosphate glucose pyrophosphorylase （AGPase） anti-sense gene under the drive of CaMV35S promoter, the plasmid pBAGP was transferred into tomato （Lycopersicon esculentum Mill） cultivar ＂Hezuo908＂ via agrobacterium-mediated transformation. The results of anti-kanamycin test, PCR amplifications of AGPase gene and NPT 11 gene and PCR-southern blotting proved that AGPase anti-sense gene was successfully integrated into genome of tomato, which provided a material basis for improving tomato breed.