仿刺参横切再生的研究

The regeneration when sea cucumber Apostichopus japonicus was transversally cut into pieces

以旅顺龙王塘海区自然生长的仿刺参Apostichopus japonicus为研究材料,将仿刺参放入含0.54 mol/LMgSO4的海水中麻醉30 min,然后用手术刀从麻醉仿刺参身体的前1/3处、后1/3处和身体中间完全横切,切后放入正常海水中饲育,定期取样观察仿刺参的消化道和体壁形态学的变化。结果表明:仿刺参横切后1 h,体壁伤口便开始收缩,部分个体将体内剩余的消化道由体壁断面处排出体外;切后第6 d,体壁切口基本愈合;切后第20 d,排出消化道的仿刺参开始再生出新的消化道;再生出具有摄食与排泄功能的完整个体大约需要100 d。

Sea cucumber （Apostichopus japonicus） with body length of 15 -20 cm were collected from Longwangtang offshore in Dalian coast, relaxed in seawater containing 0.54 mol/L MgSO4 for 30 minutes, and then transversally cut at the 1/3 anterior, 1/3 posterior and 1/2 body length. The cut sea cucumber were reared in natural sea water and the regeneration of the alimentary canal, and body wall was observed by a morphological method. The resuits showed that the sea cucumber started to shrink in the wound 1 hour after cut. Some sea cucumber eviscerated the rest of the viscera from the cut body walls. The cut body walls were healed up 6 days after the animals were cut. The cut sea cucumber eviscerated regenerated new alimentary canal on the 20th day after cut and had an integrity animal which functioned as feeding and excreting about 100 days after cut.