聚合酶链反应在结核病诊断中的应用

APPLICATION OF POLYMERASE CHAIN REACTION(PCR) IN TUBERCULOSIS(TB) DIAGNOSIS

对195例临床可疑结核病经常规培养和涂片抗酸染色阴性的标本行PCR技术检测。结果51例结核杆菌DNA呈阳性（26．2）144例阴性。阳性患者行抗结核治疗有效；阴性患者随访50例．未见假阳性、假阴性。提示应用PCR检测结核杆菌较常规方法具有准确、灵敏的特点，在结核病诊断中有重要意义。

Through the examination of polymerase chain reaction (PCR ) in the specimens of 195 cases. who are suspicious in suffering from tuberculosis (TB ) and negative both in routine culture and smear acid-fast staining .the authors find that Th DNA is positive in 51 cases (26.2%) .and negative in other 144 cases. The positive cases are effective after formal anti-TB treatment; and no false positive or negative case is found in all 50 following-up cases. The data Suggests that it is accurate, sensitive and has very important value using PCR to examine mycobacteria tuberculosis.