类胰岛素生长因子_1促腱细胞生长的实验研究

Insulin-like growth factor induces human fetal tenoeyte proliferation:an experimental study

探索促进肌腱细胞增殖的方法。方法：将原代人死胎肌腱细胞和第６代，第１３代冻存后复苏的人胎儿肌腱细胞进行体外培养，并在培养基中加入不同浓度的类胰岛素生长因子１。通过绘制生长曲线％３Ｈ－ＴｄＲ渗入等方法，观察ＩＧＦ－１对肌腱细胞的作用。结果：ＩＧＦ－１能使培养的肌腱细胞增殖加快，提前进入平顶期。

Objective:To investigate whether insulin-like growth factor_1(IGF_(-1)) induces the proliferation of human fetal tenocyte. Methods:The primary tenocytes of dead human fetus and the reanimated 6th and 13th generation tenocytes were incubated with IGF_(-1) at different concentrations. The influence of IGF_(-1) on tenoeyte was observed by means of growth curve and 3~H-TdR permeation. Results:The tenocyte incubated with IGF_(-1) at the concentration of 5μg/L reached the stagnate phase one day earlier than the control,There was dose-effect relationship between IGF_(-1) concentration and tenocyte proliferation at concentration of 1.25μg/L to 40μg/L. Tenocyte proliferation increased in a dose dependent fashion after incubation with IGF_(-1). Furthermore,IGF_(-1) stimulated hyperplasia but not hypertrophy in this in vitro system. Conclusion: IGF_(-1) has an obvious effect in promoting human fetal tenocyte proliferation.