结核杆菌快速培养、耐药性的临床应用研究

Application of mycobacteria quick colouring liquid medium and quick allochroic drug-sensitive test

目的:采用快速变色液体培养基进行结核杆菌培养及药物敏感性试验,建立一种适合基层实验室使用的快速培养及药敏试验方法。方法:①疑似肺结核痰标本200例镜检后分别接种于变色培养基和L-J培养基培养并观察结果。②35株结核杆菌采用变色培养法与罗氏绝对浓度法,65株结核杆菌采用变色培养法与罗氏比例法,分别进行药物敏感性试验。结果:变色培养基培养阳性率、污染率与L-J培养基无显著性差异,培养阳性时间明显缩短;变色培养法与绝对浓度法药敏结果相比,符合率为60%,与比例法药敏结果相比,符合率为56.9%,药敏试验培养时间显著缩短。结论:采用快速变色培养基进行培养及药敏试验,提高了分枝杆菌快速检测水平,具有较好的基层推广价值。

Objective：Using quick colouring liquid medium to do bacillus tuberculosis culture and susceptibility test ,to establish a rapid, sensitve, specific and low-cost method of rapid culture and drug-sensitive test for basic level lab. Methods：（1） Sputum samples of 200 tuberculosis-like patients were inoculated to quick coloring liquid medium and L-J medium respectively after microscope inspection by thick smearing Acid-Fast stain . （2）35 strains of tuberculosis were applied by method of quick,liquid culture and concentration method of L-J,65 strains were applied by method of quick, liquid culture and proportion method of L-J to detect their susceptibility of SM, INH, RFP and EB.Results： For postive rate and contamination rate,there was no marked difference between quick colouring liquid medium and L-J medium （P〉 0.05）,but there was a marked difference in the two group of data on average growth time（P〈0.05）;The results of susceptibility test by method of quick,liquid culture and concentration method of L-J show that coincidence is 60%, The results of quick,liquid culture and proportion method show that coincidence is 56.9%. Average growth time of drug-sensitive test is significantly shorten than routine test.Conclusion：It can improve the level of rapid inspection to use mycobacteria quick colouring liquid medium to do mycobacteria culture and drug-sensitive test, and has local clinical application and generalizing value.