高效液相色谱法测定蒙药草乌花中药效组分的含量

Determination of the Active Components Alignment in Flos Aconiti Kusnezoffii by HPLC

目的:建立以高效液相色谱法同时测定蒙药草乌花中乌头碱、新乌头碱、次乌头碱含量的方法。方法:Extend-C18(250mm×4.6mm,5μm),流动相为甲醇-0.3mol/L三乙胺(65∶35),流速为0.8mL/min,检测波长为235nm,柱温为35℃。结果:乌头碱、新乌头碱、次乌头碱分别在1～5μg,0.35～1.75μg,0.5～2.5μg范围内呈良好的线性关系。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。

Objective： To establish an HPLC method for simultaneous content determination of aconitine, mesaconitine, and hypaeonitine in Flos Aconiti kusnezoffii. Methods： Extend -C18 （250mm ×4. 6mm ,5 μm） was used as chromatographic column, methanol - 0.3 mol/L triethylamine （ 65 ： 35 ） as mobile phase. The detection wavelength was set at 235 nm, the flow rate at 0. 8mL/min and the column temperature at 35℃. Results ：The sample sizes of aconitine, mesaconitine, hypaconitine had good linear relationship with their peak areas in the range of 1 - 5μg, 0.35 - 1.75μg, and 0.5 - 2.5 μg, respectively. Conclusion ：The established method is convenient and accurate with satisfactory separation effect,wide linear range, and high sensitivity ,and can be available for the quality control of Aconitum kusnezoffii Reichb.