常见胃溃疡模型小鼠胃组织病理变化及差异

The Pathological Change and Difference of the Common Used Gastrelcosis Mice Models

目的:观察常见胃溃疡模型小鼠胃的组织病理变化,评价和比较各模型。方法:以昆明小鼠为实验对象,选取水浸拘束、乙酸、磷酸组胺、消炎痛、利血平、乙醇等6种方法,同步制作胃溃疡模型,并设置正常、禁食、假手术等对照组。在造模当天(第1天)及第8天取胃组织作病理组织学观察。结果:禁食、水浸拘束、假手术等造模当日,小鼠胃出现明显的病理变化,第8天基本消失,持续时间短;消炎痛、利血平、乙醇造模当日,小鼠胃出现明显的病理变化,至第8天其病理改变仍未完全消失;磷酸组胺病变程度轻且易于恢复;乙酸造模对动物损伤重,易致死亡。以上病理变化,大多为黏膜局部或弥漫性炎症、水肿、出血,或黏膜萎缩、坏死和脱落,属急性胃炎或部分急性胃溃疡性质。结论:水浸拘束、消炎痛、利血平、乙醇等造模成模率高,假手术、禁食次之,而磷酸组胺和乙酸造模似不宜作为小鼠常规胃溃疡模型。

Objective ：To observe the pathological change of the common used gastric ucer mice model,to appraise and compare these various models. Methods ： We select six methods ： stress, acetic acid, histamine phosphate, antifani, apoplon,alcohol to make gastric ulcer model simultaneously, and set up the normal, abrosia, and sham operated control groups. We get the stomach to observe pathohistology on the 1 st and 8th day. Results ：On the 1 st day, there were conspicuous pathological changes in the abrosia, stress and sham operated groups mice stomach which disappeared on the eighth day. In the antifani, apeplon and alcohol groups obvious pathological changes were found on the 1 st day and lasted till the 8th day. The histamine phosphate group＇s pathological changes were slight and easy to recover. The acetic acid seriously damaged the animal and easily to resulted in death. The Above mentioned pathological changes, which were mostly mucous membrane partial or diffuse inflammation, dropsy, hemorrhage, or the mucous membrane atrophia, the necrosis and amotic, were the acute gastritis or the partial acute gastric ulcer. Conclusion ： The stress, antifani, apeplon, and alcohol model highly successful ratio. The abrosia and sham operated control groups were the next,but the histamine phosphate and the acetic acid group seem not to suit the mouse conventional gashtric ulcer model.