摘要
目的：采用柱层析——分光光度法测定6批广藿香叶中总黄酮的含量。方法：以芦丁作参照物，在室温25℃下，510nm处测定广藿香叶中总黄酮的吸光度。结果：该方法在线性10．1～50．5μg／mL范围内，r=0．9996，平均回收率为97．2％～98．3％；RSD≤2．0％。结论：该方法测定广藿香叶中总黄酮，方法简便，灵敏度和准确度高。
Objective ：Content of total flavoniod in leaves ofPogostemon cablin （Blaneo） Benth. was determination by spectrophotometry. Methods ：The detection was set at 510nm and temperature at 25℃. Results ：The method was linear within the range of 10. 1 - 50.5mg/mL, r = 0. 9996. The average recovery of assay was 97.2%- 98.3% （ n = 5） and RSD ≤2.0% with reference Rutin. Conclustion ：The mothod appeared to be simple, highly accurate. It can be useed for the determination of total flavonid.
出处
《中华中医药学刊》
CAS
2008年第3期602-603,共2页
Chinese Archives of Traditional Chinese Medicine
基金
沈阳市科技局资助项目沈科发[2006]48号(1063309-1-00)
关键词
紫外分光光度法
广藿香叶
总黄酮
芦丁
spectrophotometry
Leaves of Pogostemon cablin （Blaneo） Benth
Total Flavonoid
Rutin
