尼古丁给药和撤药模型的制备与评价

Establishment and evaluation of a nicotine-treatment and-withdrawal rat model

目的：制备一种尼古丁给药和撤药大鼠模型,通过三项相关指标评价该模型的特征与应用研究价值。方法：10~11周龄的雄性SD大鼠随机分为生理盐水组、尼古丁给药组和尼古丁撤药组3组。生理盐水组给生理盐水6周;尼古丁给药组给尼古丁（3mg·kg-1·d-1）6周;尼古丁撤药组先给尼古丁（3mg·kg-1·d-1）3周,随后改为给生理盐水3周。各组给药途径均为皮下注射,每日1次,给药容积为0.5ml/kg,同时记录体质量、饮食量和饮水量的变化。造模后通过体质量、血清生化指标、各部位脂肪称量来评价该模型。结果：尼古丁给药后体质量下降;血清三酰甘油、胰岛素水平降低;皮下脂肪、内脏脂肪和主动脉周围脂肪质量减少;撤药后上述指标回升。结论：该模型可用于研究尼古丁的多种药理作用,以及吸烟、戒烟对机体的影响。

Objective：To establish a nicotine-treatment and -withdrawal rat model and to evaluate its characteristics and application through analyzing 3 parameters. Methods：Male Sprague-Dawley rats, aged 10-11 weeks old, were randomly divided into normal saline group（subcutaneous injection of saline [0.5 ml/kg] for 6 weeks）, nicotine-treated group （injection with nicotine [5 ml/kg, 3 mg/（kg · d）] for 6 weeks）, and nicotine-withdrawn group （injection with nicotine [0.5 ml/kg, 3 mg/（kg · d）] for 3 weeks and followed by saline injection for additional 3 weeks）. Body weight, food intake, and water intake of animals were recorded during the treatment in 3 groups. The model was evaluated through analyzing body weight, serum parameters and adipose tissue weights. Results：The body weight of rats, as well as the serum levels of triglyceride and insulin, were all decreased after nicotine treatment; the weights of subcutaneous fat, visceral fat and periaortic fat were also decreased. The above indicators increased after withdrawal of nicotine. Conelusion：The established model can be used to study multiple pharmacological effects of nicotine; it can also be used for smoking and smoking cessation related studies.