贵金属合金及粗糙度对种植体龈下优势菌黏附性的影响

The effect of different roughness of crown alloy materials on the adhesion of predominant peri-implant subgingival bacteria

目的:观察2种合金及其粗糙度对种植体龈下优势菌黏附性的影响。方法:选用2种常用冠修复合金材料:金铂合金和金钯合金。将其制成2种不同粗糙度的试件,粘固于种植纯钛板上,分别与4种龈下优势菌Pg、Aa、Pi及Fn共同厌氧孵育,采用菌落形成单位(CFU)计数法量化测定培养试件表面的细菌黏附量。应用SPSS13.0软件包,对试件表面粗糙度的测量值及试件上黏附的菌落数进行统计学分析,同时在扫描电镜下观察各种菌在试件上的附着情况。结果:同种合金中,粗糙度大的试件4种细菌附着量均大于粗糙度小者(P<0.01);同种粗糙度的试件,金铂合金与种植体钛上各种细菌黏附量均大于金钯合金与种植钛上的附着量(P<0.01)。结论:金钯合金对细菌黏附的影响比金铂合金大,同种合金粗糙度大者,细菌黏附量多。

PURPOSE： To investigate the effect of two crown metal alloys with different roughness on the adhesion of predominant subgingival bacteria. METHODS： Au- Pt alloy and Ni-Cr alloy with different roughness were attached to the Ti implant material and incubated under anaerobic condition. The adhesion of four subgingival predominant bacteria： Porphyromanus gingivalis（Pg）, Actinobacillus actinomycet-emcomitans （Aa）, PrevoteUa intermedia （Pi） and Fusobacterium nuclertum （Fn）, was detected, respectively. The quantity of bacteria clones on the different surface was counted by means of clone forming unit （CFU） method, and the shape and the growth characteristics of adhered bacteria were observed under scanning electron microscopy （SEM）. The date was analyzed using SPSS13.0 software package. RESULTS： The amounts of the four subgingival predominant bacteria adhered to rougher surface of same alloy samples increased significantly （P〈 0.01）, and the amount of bacteria adhered to the surface of Au- Pt alloy samples was much more than that to alloy Au-Pb samples with the same roughness （P〈0.01）. CONCLUSIONS： The effect of Au-Pt alloy is significantly greater than Au- Pb alloy to the adhesion of bacteria. The amount of bacteria adhered to rougher surface of same alloy samples is more than those to smooth one.