新生大鼠海马神经干细胞表达趋化因子受体CCR2的体外研究

In Vitro Study about Expression of Chemokine Receptor CCR2 in Neural Stem Cells of Neonatal Rat Hippocampi

目的探讨趋化因子受体CCR2在体外培养神经干细胞的表达.方法采用无血清方法分离、培养新生大鼠海马神经干细胞,细胞免疫荧光方法检测神经干细胞标志巢蛋白(nestin)表达及干细胞多向分化为神经元及胶质细胞的能力,通过细胞免疫荧光及RT-PCR的方法检测神经干细胞表达趋化因子受体CCR2的情况.结果体外培养新生大鼠海马神经干细胞呈nestin阳性,可分化为NF200阳性神经元、GFAP阳性星形胶质细胞及CNP阳性少突胶质细胞,CCR2细胞免疫荧光及RT-PCR证实神经干细胞表达趋化因子受体CCR2.结论新生大鼠海马神经干细胞表达趋化因子受体CCR2,为进一步研究其体内、外迁移提供理论依据.

Objective To explore whether neural stem cells, in vitro, expressed chemokine receptor CCR2. Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in serum-free medium. By using immunofluorescence, the neural stem cells and its differentiation into neuron and glial cell types were identified. Then immunofluorescence and RT-PCR were employed to detect whether neural stem cells, in vitro, expressed chemokine receptor CCR2. Results The floating neural spheres, isolated from neonatal hippocampi, were nestin positive and could differentiate into cells to express characteristic antigen of neurons, astrocytes and oligodendrocytes. Immunofluorescence and RT-PCR showed neural stem cells expressed chemokine receptor CCR2. Conclusion Neural stem cells, in vitro, expressed chemokine receptor CCR2, which could offer theoretical evidence for further study of its migration.