多发性硬化患者外周血Annexin V 结合T淋巴细胞凋亡和Fas的表达

Annexin V Blinding Fas-mediated Apoptosis of T Lymphocyte and the Expression of Fas on T Lymphocyte in Peripheral Blood of Patients with Multiple Sclerosis

目的探讨多发性硬化(MS)患者外周血T淋巴细胞凋亡和Fas表达.方法42例复发缓解型MS患者,根据其病程分为复发期组20例和缓解期组22例,另外选择20例健康人作为对照组,采用五色流式细胞术(FCM)检测以上3组外周血T淋巴细胞凋亡和Fas表达的情况.结果3组间外周血T淋巴细胞Fas的表达无明显差别;复发期MS患者凋亡的CD4+T淋巴细胞(由AV染色标记)和Fas相关的CD4+T淋巴细胞凋亡(由AV+CD95+/CD4+标记)明显低于缓解期MS患者和对照组(P<0.05);虽然复发期MS患者和缓解期MS患者凋亡的CD8+T淋巴细胞(由AV染色标记)和Fas相关的CD8+T淋巴细胞凋亡(由AV+CD95+/CD8+标记)都明显低于对照组(P<0.05),但复发期MS患者和缓解期MS患者之间无明显差异(P>0.05).结论T淋巴细胞的凋亡和Fas的表达可能与复发缓解型MS患者的发病和病情有关.

Objective To explore the potential relationship among expression of Fas （CD95） on T lymphocyte, Fas-mediated apoptosis and the disease course of multiple sclerosis （MS） Methods Forty-two relapsing-remitting MS patients were divided into active MS group （20 patients） and inactive MS group （22 patients） , and twenty healthy subjects were selected as controls. Annexin V （AV） blinding and Fas expression on T cells in peripheral blood from MS patients and controls were examined in all groups by five-color flow cytometry. Results The percentage of AV^＋CD4^＋CD3^＋ cells （defined by AV） and CD95^＋AV^＋CD4^＋CD3^＋ cells （defined by CD95 and AV） in peripheral blood significantly decreased in active MS patients compared with those in inactive MS patients and control. The percentage of AV^＋CD8^＋CD3^＋ cells （defined by AV） and CD95^＋AV^＋CD8^＋CD3^＋ cells （defined by CD95 and AV） in peripheral blood significantly decreased in active MS patients and inactive MS patients compared with those in the controls. Conclusions These results indicate that the resistance of CD4^＋T cells to Fas-mediated apoptosis is involved in exacerbation of MS and/or that Fas-mediated apoptosis of T cells is associated with remission of MS.