基质金属蛋白酶2在鼻咽癌组织中的表达及其与肿瘤细胞生物学行为的关系

Expressive activity of matrix met alloproteinase-2 in nasopharyngeal carcinoma tissues and its implications in malignent biological behavior

目的研究基质金属蛋白酶2(MMP2)在鼻咽癌组织中的表达及其与鼻咽癌细胞生物学行为的关系。方法采用免疫组化Elivision Plus二步法对50例鼻咽癌组织、15例炎性鼻咽黏膜组织的MMP2表达活性进行检测与比较。结果鼻咽癌组织中的MMP2表达显著高于炎性鼻咽黏膜组织(P<0.05),并随鼻咽癌临床浸润分期的升高和淋巴结转移的发生而上调,差异具有统计学意义(P<0.01)。结论MMP2在鼻咽癌细胞突破基底膜向外扩散及转移中可能起着重要作用,对于判断鼻咽癌细胞的侵袭性及估计其预后中有一定意义。

Objective To investigate the expressive activity of matrix met alloproteinase-2 （MMP-2） in the tissues of nasopharyngeal carcinoma （NPC） and its implications in the biological behavior of the tumor based on a study carried out in a group of clinical samples. Methods The expressive activity of MMP-2 was determined by immunohistochemical Elivision Plus methods in 50 cases of NPC tissues and 15 tissue samples of inflammatory nasopharyngeal epithelia to analyze the expressive difference in various tissues and its implications in the biological behavior of the tumor. Results The Positive rate of MMP-2 was significantly higher in N-PC tissues than that in inflammatory tissues （P〈 0.05） and was significantly relevant with the infiltrating grading in local tissue and lymphatic metastasis rate in the neck. Conclusions MMP-2 may Play a critical role in the Pathological Process of tumor cells to degrade the basement membrane to in vade surrounding tissues and to form metastatic lesions in neck lymph nodes. Determination of MMP-2 activity in the tumor tissues should be of some significance in Predicting the invasion Potentiality of NPC cells and in the evaluation on the Prognosis of such cases.