大鼠胼胝体神经元表达Cav，2.2及其神经纤维联系

Neurons in the corpus callosum of rats:expression of Ca_v2.2 and their connection

目的:证明成年大鼠胼胝体内存在神经元,检测胼胝体神经元是否表达钙通道蛋白并追踪胼胝体神经元纤维联系方式。方法:选取正常SD大鼠脑,冰冻连续切片进行成熟神经元的标志物NeuN和N型电压依赖性钙通道α1亚单位(Cav2.2)的免疫组织化学染色,应用神经束路逆行追踪剂30%辣根过氧化物酶(HRP)生理盐水溶液注射入胼胝体小钳下方,对HRP进行四甲基联苯胺(TMB)显色。分别观察胼胝体中NeuN和Cav2.2的表达和胼胝体内神经元突起的联系情况。结果:在胼胝体中存在少量NeuN阳性细胞,部分NeuN阳性细胞同时表达Cav2.2。神经逆行追踪显示在胼胝体内存在HRP阳性神经元,其神经纤维连接与皮质或尾壳核有联系。结论:在成年大鼠胼胝体内存在少量的神经元,部分胼胝体神经元表达Cav2.2。这些神经元可能与脑皮质和胼胝体下方的尾壳核有联系。

Objective To prove the existence neurons in the rat corpus callosum, the potential function of these neurons and their connection. Methods Immunohistochemistry was used performed to examine the expressions of NeuN, a mature neuron marker, and N-type voltage-dependent valcium channel α1 -subunit （ Ca2.2 ） in the section of the rat corpus callosum. Horseradish peroxidase （ HRP ） normal sodium solution （ 3 0 % ） , the retrograde tracer, was injected under the frontal forceps of corpus callousm and HRP absorbed by the process of neurons in the brain slices was stained with tetramethyl benzidine. Results There were some NeuN positive cells in the rat corpus callosum and Cav2.2 was detected in some of these NeuN positive cells. Neurons with positive HRP were found in the rat corpus callosum and some of these neurons connected to the cortex or corpus striatum. Conclusion There are a few neurons in the corpus callosum of adult rats and some of them express Cav 2.2. Neurons in the corpus callosum have connections with the brain cortex or corpora striatum.