COX-2抑制剂对乳腺癌放射增敏作用的实验研究

Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer

目的:探讨环氧化酶2抑制剂尼美舒利对乳腺癌细胞株MCF-7的放射增敏作用并初步探讨其机制。方法:克隆形成实验检测尼美舒利对MCF-7的放射增敏作用;电镜观察肿瘤细胞形态学改变;流式细胞仪(FCM)检测肿瘤细胞调亡率及Bcl-2、Bax蛋白的表达。结果:不同浓度的尼美舒利对乳腺癌细胞MCF-7均有放射增敏作用,且呈剂量依赖性关系;尼美舒利与射线照射联用能够上调Bax的蛋白表达,同时抑制Bcl-2蛋白表达,明显增加细胞凋亡率。结论:COX-2抑制剂尼美舒利对人乳腺癌MCF-7细胞株具有明显的放疗增敏作用。

Objective：To investigate the radiosensitivity enhancement effect of COX-2 inhibitor -Nimesulide on breast cancer cell line（MCF-7） in vitro and to initially disclosure its mechanism. Methods：The clonogenic assay was performed to determine the radiosensitizing effect of Nimesulide on MCF-7 cell line. The morphological changes of MCF-7 were observed by electron microscopy. The apoptosis and expressions of Bcl-2 and Bax were measured by flow cytometry （FCM）. Results： Nimesulide at different concentration significantly enhanced the radiosensitivity of MCF-7 cells, in a dose-dependent manner, under the combined treatment of Nimesulide and radial the protein expression of Bax was up-regulated, while that of Bcl-2 was inhibited and the apoptpsis increased significantly. Conclusion：Cox-2 inhibitor could obviously enhance the radiosentivity of human breast cancer cell line MCF-7.