一种新的前循环蛛网膜下腔出血动物模型

A new animal model of subarachnoid hemorrhage in the anterior circulation

目的:建立一种简便、重复性好的蛛网膜下腔出血(SAH)动物模型。方法:在立体定位下将导管插入视交叉前池,注入300μl自体动脉血建立SAH模型。监测动脉血压、血气分析、颅内压,采用激光多普勒血流量仪(LDF)测定局部脑血流量(rCBF)和进行神经行为学评分。结果:血液广泛分布在前颅窝底、Willis环、基底池周围。SAH组大鼠的血压、血pH、PaO2、PaCO2值之间差异无统计学意义(P>0.05)。ICP在SAH后1min迅速增高至最大值(P<0.05)。rCBF在SAH后1min迅速降低,于SAH后3min进一步降低至最低点(P<0.05)。大部分大鼠在SAH后24h有轻度的神经行为异常,在SAH后48h恢复正常。结论:该模型能更好地模拟临床SAH,重复性好,更适合于前循环动脉瘤性SAH后病理生理学机制的研究。

Objective：To establish a simple and reproducible animal model of subarachnoid hemorrhage （SAH）. Methods：The SAH model was induced by stereotaxic insertion of a cannula through an artifical hole into the prechiasmatic cistem and injection of 300μl autologous arterial blood. The arterial blood pressure,blood gases analysis,and intracranial pressure were monitored,and regional cerebral blood flow（rCBF） was assessed by laser Doppler flowmetry（LDF）. A neurobehavioral evaluation was performed at 24 and 48h after SAH. Results：The subarachnoid blood was widely distributed throughout the anterior cranial fossa base,Willis circle and basal cistemal system. There was no statistically significant difference between BP,pH,PaO2 or PaCO2 values of the SAH group and those of the control group（P〉0.05）. ICP increased rapidly to the maximum lmin after SAH（P〈0.05）. rCBF decreased rapidly in lmin after SAH,and continued to fall to its nadir in 3min later（P〈O.05）. Slight neurobehavioral disorders were showed for most rats subjected SAH in 24h,but normalized in 48h after SAH. Conclusion：This new model resembles clinical SAH. It is very reproducible and appropriate for the study on the pathophysiological mechanisms of aneurysmal SAH in the anterior circulation.