摘要
目的:观察同源盒蛋白(Msx1)过度表达在成骨细胞MC3T3-E1分化培养过程中对碱性磷酸酶的调节作用,以探讨Msx1蛋白对细胞成骨分化过程的调控机制。方法:将成骨细胞MC3T3-E1分为5组:未转染病毒组作为对照组1(A组);空白腺病毒载体组作为对照组2(B组);未分化组作为空白对照组(C组);分化前1 d转染携带同源盒基因Msx1的腺病毒载体组(D组);分化后1 d转染Msx1腺病毒载体组(E组)。在成骨分化培养基中培养4 d后,检测成骨细胞MC3T3-E1在成骨分化培养过程中碱性磷酸酶(alkaline phosphatase,ALP)的活性。结果:A、B两组成骨细胞MC3T3-E1在成骨分化培养基培养4 d后,ALP活性呈强阳性;C组中未分化成骨细胞MC3T3-E1无ALP活性,D、E两组的成骨细胞MC3T3-E1在成骨分化培养4 d后的ALP活性明显较A、B组减低,而且D、E两组间ALP活性无区别。结论:过度表达同源盒蛋白(Msx1)能抑制成骨细胞MC3T3-E1的ALP表达,在其成骨分化的过程中起一定的抑制作用。
Objective： To observe the effect of overexpression of homeoprotein Msx1 on expression of alkaline phosphatase （ALP）, and study Msx1 regulation mechanism during the process of osteoblasts differentiation, Methods： Osteoblasts MC3T3-E1 were divided into 5 groups： non-transformed osteoblasts as control 1（group A）; osteoblasts transformed by blank adenovirus vector as control 2（group B）; non-differentiation osteoblasts as negative control （group C）; osteoblasts transformed by Msx1 adenovirus 1 day before being cultured in osteogenic differentiated-medium （group D）; osteoblasts transformed by Msx1 adenovirus 1 day after being cultured in osteogenic differentiated-medium （group E）, ALP activities were tested after being cultured in osteogenic differentiated-medium for 4 days, Results： Strong positive signals were detected in groups A and B. There was no activity of ALP detected in group C. ALP activities in groups D and E, in which osteoblast MC3T3-E1 overexpressed homeoprotein Msxl, were obviously decreased without difference between each other, Conclusion： Overexpression of homeoprotein Msxl can inhibit ALP activity of osteoblasts and may plays a role in the differentiation process of osteoblasts.
出处
《口腔颌面外科杂志》
CAS
2008年第1期19-22,共4页
Journal of Oral and Maxillofacial Surgery
