摘要
目的探讨过量碘对甲状腺滤泡上皮超微结构的影响，从而探讨甲状腺损伤与自身免疫甲状腺炎之间的关系。方法NOD．H-2^h4和昆明小鼠随机分入饮用正常水组和5倍、10倍、100倍高碘水组。各组动物分别于实验开始后4、8及24周处死，锇酸固定，醋酸双氧铀和柠檬酸铅双重染色，透射电镜成像。结果碘过量造成NOD．H-2^h4小鼠甲状腺上皮细胞过氧体和次级溶酶体数量增多、上皮细胞凋亡、坏死、滤泡结构破坏和炎细胞的浸润，而且这种损伤与碘剂量呈正相关。结论碘过量对甲状腺上皮细胞的氧化损伤作用可能是自身免疫甲状腺炎产生的必要条件。
Objective To investigate the effect of iodine excess on thyroid follicle epithelial ultrstructure and the relationship between thyroid injury and autoimmune thyroiditis. Methods NOD. H-2^ht mice and Kunming mice were randomly divided into four groups receiving plain water, 5 fold, 10 fold, and 100 fold excessive iodine water. 4, 8 and 24 weeks after receiving iodine water, the mice were killed. After fixation with osmic acid and dual staining with uranyl chloride and citrate lead, thyroid gland ultrstructure was examined with electron microscopy. Results Iodine treated NOD. H-2^ht mice exhibited marked accumulation of peroxisome and secondary lysosomes, apoptosis and necrosis of thyroid epithelial cell, damage of thyroid follicles and lymphocytic infiltration. The observed changes induced by iodine were in a dose dependent way. Conclusion The oxidative injury on the thyroid epithelial cells induced by iodine excess might be the prerequisite for the creation of autoimmune thyroiditis.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2008年第3期193-196,共4页
Chinese Journal of Internal Medicine
基金
国家自然科学基金（30240013
30370680）
