细胞周期抑制蛋白p16^(INK4a)在宫颈病变中的异常表达

Abnormal Expression of Cell Cycle Control Protein p16^(INK4a) in Cervical Lesions

[目的]探讨宫颈癌(UCC)、宫颈上皮内瘤变(CIN)及宫颈炎中p16INK4a蛋白表达水平的变化及意义。[方法]应用免疫组化方法检测126例宫颈病变(UCC50例、CIN57例和宫颈炎19例)组织中p16INK4a蛋白的表达,进行半定量分析及统计学检验。[结果]50例UCC中,p16INK4a全部阳性表达;57例CIN中,p16INK4a阳性表达41例,阳性表达率为71.93%,其中CINⅠ,CINⅡ,CINⅢp16INK4a阳性表达率分别为36.84%(7/19)、81.82%(18/22)、100.00%(16/16),且三者间差异有显著性(P<0.05)。19例宫颈炎组织中,p16INK4a阳性表达8例,阳性表达率为42.11%。p16INK4a蛋白在宫颈炎、CIN和UCC组织中表达水平依次升高,且差异有显著性(P<0.05)。[结论]UCC及癌前病变中p16INK4a阳性表达明显高于良性病变。p16INK4a在UCC及癌前病变中过表达,提示其在UCC发生、发展中起重要作用。

[Purpose] To study the expression of p16^INK4a protein in uterine cervical cancer （UCC）, cervical intraepithelial neoplasia （CIN） and cervicitis, and its significance. [Methods] The expression of p16^INK4a protein was detected in 126 cervical samples （UCC 50 cases, CIN 57 cases, cervicitis tissue 19 cases） by immunohistochemical staining. [ Results ]p16^INK4a protein was positive in all 50 cases with UCC. The positive expression of p16^INK4a was detected in 41 of 57 cases with CIN （71.93%）. The positive rates of CIN Ⅰ, CIN Ⅱ, CINⅢ samples were 36.84% （7/19）, 81.82 % （18/ 22）, 100.00% （16/16） respectively with significant difference among the three groups （P〈0.05）. The positive expression of p16^INK4a was detected in 8 of 19 cervicitis cases （42.11%）. p16^INK4a increased with an increasing order in cervicitis, CIN to UCC, with significant difference among the 3 groups（P〈0.05）. [Conclusionl p16^INK4a overexpresses in UCC and precancerous lesions, which plays an important role in carcinogenesis of UCC.