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槲皮素对人膀胱癌BIU-87细胞增殖和凋亡的影响 被引量：14

The Effect of Quercetin on the Proliferation and Apoptosis of Human Bladder Cancer Cell BIU-87
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摘要 目的研究槲皮素(Quercetin)在体外对人膀胱癌BIU-87细胞增殖和凋亡的影响。方法对在体外经槲皮素不同浓度和时间处理后的人膀胱癌BIU-87细胞,分别采用MTT试验检测细胞增殖的变化,流式细胞技术和倒置显微镜分析细胞周期及凋亡。结果MTT试验显示槲皮素能抑制人膀胱癌BIU-87细胞生长,且呈一定的剂量和时间依赖性;流式细胞仪检测发现,一定浓度和时间的槲皮素作用BIU-87细胞后能阻滞G2/M期细胞向G0/G1和S期移行,且在细胞周期G1峰的左侧出现了亚G1凋亡峰;倒置显微镜观察发现有凋亡的特征性改变。结论槲皮素能抑制人膀胱癌BIU-87细胞增殖,阻滞细胞于G2/M期,并诱导细胞凋亡。 Objective To study the effect of quercetin on the proliferation and apoptosis of the human bladder cancer cell BIU-87. Methods After treated with different concentrations of quercetin in vitro for different times, the proliferation of BIU- 87 cells was evaluated by means of MTT, and cell cycle and apoptosis were observed by flow cytometry and inverted microscopy. Results Quercetin inhibited proliferation of BIU-87 cells,and this effect was dose and time dependent. Flow cytometry study showed that quercetin could inhibit the BIU-87 cells entering G0/G1 ＋ S from G2/M phase. Characteristic apoptotic changes was observed under inverted microscope after treated with certain concentrations of quercetin and time. Conclusion Quercetin can inhibit proliferation,induce apoptosis and block the cell cycle at G2/M of human bladder cancer BIU-87 cells.
作者 柯尊金 丁心喜 董文奎 王慕义 朱伟 曾甫清
机构地区 湖北省老河口市第一医院泌尿外科 华中科技大学同济医学院附属协和医院泌尿外科
出处 《实用癌症杂志》 2008年第2期116-118,共3页 The Practical Journal of Cancer
关键词 槲皮素 膀胱癌 增殖抑制 凋亡 Quercetin Bladder cancer Inhibited proliferation Apoptosis
分类号 R737.14 [医药卫生—肿瘤]
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参考文献9

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二级参考文献32

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实用癌症杂志
2008年 第2期

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