人血管内皮细胞在白血病小鼠中参与形成微血管的研究

Study of Human Endothelial Cell on Construction of Microvessel Angiogenesis in Leukemia Mice

【目的】研究人血管内皮细胞在建立NOD/SCID小鼠白血病微血管中的作用。【方法】NOD/SCID小鼠分3组:A空白对照组,B注射EC组,C建立白血病模型后注射EC组。通过免疫组化对NOD/SCID小鼠骨髓的血管生成及全身肿瘤负荷情况进行评价。【结果】C组骨髓微血管密度明显大于A、B组,白血病新生的微血管分支多紊乱,管腔较不规则,血管形态幼稚。【结论】用人血管内皮细胞成功建立NOD/SCID小鼠白血病微血管生成模型,可用于抗白血病血管生成及相关实验的研究。

[Objective] To study the role of human vascular endothelial cell in angiogenesis in NOD/SCID leukemia mice. [Methods] NOD/SCID mice were divided into 3 groups： A, blank group; B, EC injection group; C, leukemia model mice with EC injection group. MVD in bone marrow with cnemis slice were calculated after immunohistochemistry dyeing. Leukemia burden was evaluated in leukemia model mice. [Results] Bone marrow MVD in group C was significantly higher than that in group B. Microvessels in leukemia mice had disordered branches and irregular cavity. The morphism of vessels was immature. [Conclusion] Microvessel angiogenesis model in NOD/SCID leukemia mice has been constructed successfully with human endothelial cell. It can be used in the study of anti-angiogenesis in leukemia and relative researches.