钝顶螺旋藻多糖抗单纯疱疹病毒2型的作用

Antiviral Activities of Polysaccharide from Spirulina Platensis against HSV-2 in Vitro

【目的】探讨钝顶螺旋藻多糖(PSP)抗单纯疱疹病毒2型(HSV-2)的作用及可能机制,为进一步开发该药提供理论依据。【方法】实验选用HSV-2标准株(333株),以不同剂量的PSP作用于HSV-2复制周期的不同阶段,终点以病毒半数感染量(TCID50)、细胞病变效应(CPE)、MTT染色细胞保护率(MTT法)及荧光定量PCR(FQ-PCR)作为评价手段,判断PSP的抗病毒效果。【结果】PSP对Vero细胞毒性极低(TC50为1750μg/mL),对HSV-2无直接灭活作用,可抑制病毒吸附和感染细胞内病毒的复制,其中50μg/mL、100μg/mL、200μg/mL浓度的PSP对病毒复制的抑制率分别为63.94%、81.41%、85.63%;FQ-PCR结果显示随着PSP浓度及作用时间的增加,PSP对HSV-2DNA的抑制作用明显增强,具有良好的剂量和时效关系。【结论】PSP在体外具显著的抗HSV-2病毒作用,此作用发生在病毒吸附、病毒基因复制等多个环节上。

[Objective] To explore the antiviral effect and mechanism of polysaccharide from Spirulina Platensis （PSP） on herpes simplex virus type 2 （HSV-2） and to provide a theoretical support for developing new drugs. [Method] A stand strain of HSV-2 （333 strain） was used to investigate the antiviral effect of PSP in vitro. PSP in various concentrations was applied to different stages of HSV-2 replication cycle. Finally, the virus infectivity （TCIDs0）, cytopathic effect （CPE） and MTr stain method for viable cells （MTF assay） were used as markers to evaluate the effect of PSP on HSV-2. The quantity of HSV-DNA was detected by real-time fluorescence quantitative PCR （FQ-PCR）. [Results] PSP had little cytotoxic effect on Vero cells （TC50=1750μg/mL）, it could not directly inactivate HSV-2 infectivity. PSP not only interfered in adsorption of HSV-2 to Vero cells but also inhibited HSV-2 replication in the cells, PSP in the concentrations of 50μg/mL, 100μg/mL, and 100μg/mL could significantly inhibit virus replication with high inhibitory rate 63.94%, 81.41%, and 85.63%, respectively. FQ-PCR results showed that the inhibitory rate on HSV-DNA also increased in a dose-dependent and time-dependent manner. [Conclusion] PSP showed good antiviral effect on HSV-2 in vitro, which may be attributed to the inhibition of virus adsorption and virus replication in the cells.