三种醛类化合物-DNA加合特性的研究

Detection of binding of three aldehyde compounds with DNA using high performance liquid chromatograph

目的探讨甲醛、乙醛和丙烯醛对DNA的损伤机制。方法采用体外测试系统，应用高效液相色谱法（HPLC）对甲醛、乙醛、丙烯醛及其在人体内的代谢产物甲酸、乙酸、羟丙基巯基尿酸与4种单脱氧核苷酸的加合反应进行研究。结果经HPLC分离检测，甲醛、醋醛与脱氧鸟苷酸发生加合反应，而丙烯醛、甲酸、醋酸和羟丙基巯基尿酸难以与脱氧鸟苷酸发生加合反应或加合物产量过低，初步确定了甲醛、乙醛与4种单脱氧核苷酸结合的优势反应核苷酸。结论甲醛、乙醛能够与DNA的脱氧鸟苷酸结合而体现遗传特性。

Objective To detect the binding sites and characteristics of the adduct from the reaction of formaldehyde, acetaldehyde, acrolein with DNA. Methods Formaldehyde,acetaldehyde, acrolein were reacted with four kinds of deoxyribonucleoside monophosphate （dNMP） in buffered solutions with neutral pH. The reaction products were separated by high performance liquid chromatograph（HPLC） and characterized by UV spectroscopy. Results The reaction of formaldehyde, acetaldehyde with dG was separated and detected by HPLC. The reaction of acrolein, formate, acetic acid, Mercapturic acid with dG wasn＇t separated and detected by HPLC, while the dominant dNMP binding with formaldehyde, acetaldehyde was also determined. Conclusion Formaldehyde, acetaldehyde could bind with dGMP to express genotoxic effects.