甲苯胺蓝为光敏剂的光动力疗法对龈上菌斑灭菌效果的研究

Study of the effect of toluidine blue(TB)-mediated photodynamic inactivation to the periodontal pathogens from supragingival plaques

目的:比较不同浓度甲苯胺蓝对龈上菌斑的灭菌效果。方法:从门诊20例牙周病病人下前牙区取龈上菌斑一份,放入10mL生理盐水中振荡均匀,在2h内送到微生物室,用微量移液器取25μL放在琼脂培养皿中,在37℃恒温箱中培养24h后,分别滴入0.01,0.1,0.5,1.0,2.5,5.0mg/mL甲苯胺蓝,用12J/cm2,159mW/cm2波长为630nm的二极管激光照射57s后,观察比较各种浓度的灭菌效果。结果:在相同的光波长、光剂量、光强度和光照时间下,1.0mg/mL的甲苯胺蓝的灭菌效果最好,灭菌率为79%。结论:以甲苯胺蓝为光敏剂的光动力疗法对龈上菌斑有灭菌作用。

AIM： To evaluate the effect of toluidine blue （TB） - mediated photodynamic inactivation to the supragingivia plaques. METHODS： Supragingivia plaques in the area of lower incisor teeth from 20 voluntary outpatients with periodontopathy. The samples were immediately placed into 10 mL pre - diluted solution and transported to the laboratory within 2 h. The 25 μL bacterial suspentions were incubated in agar broth at 37℃. After an overnight culture, the bacterial suspentions were diluted with toluidine blue （TB） at density of 0. 01,0. 1,0. 5, 1.0,2.5, 5.0 mg/mL respectively. Then they were exposed to a 12 J/cm^2 laser irradiation at 57 s . The light output of fiber was 159 mW/cm^2 from 630 nm Diode laser in order to observe the effect of inactivation to the supragingivia plaques about different groups. RESULTS： The most -effective density of TB is 1 mg/mL depends on the same light intensity, dose of light energy , wave length and irradiation time. CONCLUSION： The study have found that the toluidine blue （TB） - mediated photodynamic therapy showed of bacterium inactivation to the supragingivia plaques.