乙二醛诱导细胞凋亡的近红外表面增强拉曼光谱

Glyoxal Induced Apoptosis Studied by Near-infrared Surface-enhanced Raman Scattering

将60nm金纳米粒子导入到活的人骨肉瘤细胞中,用近红外表面增强拉曼散射(SERS)技术获取细胞内化学成分的高分辨SERS信息.对正常活性细胞和乙二醛诱导的凋亡细胞的比较研究表明,对于正常活性的细胞,金纳米探针主要分布在细胞质内(围绕细胞核),而凋亡细胞内的金纳米探针的分布较为均匀,在遍布凋亡细胞内的各个位置包括细胞表面均容易找到DNA片段的信息.

We report on the capabilities of near-infrared surface-enhanced Raman scattering（SERS） by introducing 60 nm gold nanoparticles into live human osteosarcoma cells to obtain detailed chemical information with a high spatial resolution from these cells. A comparative study between viable and glyoxal induced apoptotic cells indicates that most of the gold nanoparticles enter the living cell reside in the cytoplasm and around the nucleus, whereas apoptotic cell shows relatively uniform distribution of gold nanoparticles and interestingly, DNA fragments can be easily found throughout the cell including the cell surface. The ability to acquire very sensitive SERS inside single cells indicates the potential of this technique as a useful tool in biomedicine.