子宫内膜癌组织P^(16)基因突变及其蛋白表达的研究

Mutation of P16 gene and its protein expression in endometrial cancer tissue

目的:研究P16蛋白表达与原发性子宫内膜癌发生、发展的关系和P16基因缺失突变和点突变在原发性子宫内膜癌发生、发展中作用。方法:利用免疫组织化学方法(免疫组化法)、聚合酶链反应(PCR)和聚合酶链反应-单链构象多态性分析(PCR-SSCP)技术分别检测正常子宫内膜组织、子宫内膜癌前病变组织及原发性子宫内膜癌组织。检测P16蛋白和P16基因缺失突变和点突变。结果:①P16蛋白阳性表达率在原发性子宫内膜癌组织中明显低于正常子宫内膜组织及子宫内膜癌前病变组织;②在原发性子宫内膜癌组织中P16基因缺失突变率高于点突变率。结论:①在原发性子宫内膜癌发生、发展过程中,P16蛋白缺乏与子宫内膜细胞增殖失控及分化不良紧密相关;②原发性子宫内膜癌与P16基因缺失突变密切相关。

Objective： To investigate the relationship between expression of p^16 protein and primary endometrium cancer and to evaluate the role of p^16 gene deletion mutation and point mutation in primary endometrium carcinoma. Methods： Streptavidin - peroxidase conjugated method was performed for detection the expression of p^16 proteins in cases of normal endometrium, cases of the anterior lesion of endometrium cancer and cases of primary endometrium carcinoma, and polymerase chain reaction and polymerase chain reaction single - strand conformation polymorphism analysis for p^16 gene deletion mutation and point mutation. Results： ①The expression rate of p^16 protein in primary endometrium cancer, was much lower than that in normal endometrium and the precancerous Iesion of endometrium. ②The rate of loss mutation was higher than the rate of point mutation in primary endometrium carcinoma. Conclusion： ①In the course of generation and development of primary endometrium cancer, there is a close correction between the absence of p^16 protein and endometrium cell proliferation miss control and dysplasia. ②There is a close correction between the primary endometrium cancer and p^16 gene deletion mutation.