应用5S rRNA基因间隔鉴定川西獐芽菜和同属混淆品种(英文)

Identification of Swertia mussotii and its adulterant Swertia species by 5S rRNA gene spacer

目的：采用5S rRNA间隔序列分析区分川西獐芽菜和常见同属植物混淆品种抱茎獐芽菜，华北獐芽菜及印度獐牙菜。方法：提取獐芽菜属被测样品的DNA，使用PCR扩增5S rRNA基因间隔片段，测定及比较各品种的序列。结果．川西獐芽菜和其余3种混淆品獐牙菜属植物之间DNA的5S rRNA基因间隔序列不同，差异范围为30．6％-65．0％。结论：5S rRNA基因间隔序列可以作为分子鉴定标记区别川西獐芽菜和同属其他常见混淆品种。该方法为獐芽菜属植物的鉴定提供可靠，简便的参考依据。

Objective： This research focused on analyzing the differences of 5S rRNA gene spacer sequences on Swertia mussotii, and its commonly used adulterants, including S. franchetiana, S. wolfangiana and S. chirayita. Method： DNA was extracted from the collected Swertia samples. 5S rRNA intergenic spacers were amplified by PCR, sequenced and analyzed. Result： 5S rRNA gene spacer sequences were different between S. mussotii and its other three adulterants. Sequence divergence among species ranged from 30. 6% to 65.0%. Conclusion： 5S rRNA spacers may be used as molecular authentication markers to differentiate S. mussotii and other commonly used Swertia adulterants. This result provides reliable and simple reference for the authentication of Swertia genus species.