荭叶心通软胶囊中主要活性成分的大孔吸附树脂分离纯化工艺研究

Separation and purification technology of main active composition of Hongye Xingtong soft capsules with macroporous resin

目的:采用大孔吸附树脂法,探讨荭叶心通软胶囊中荭草、山楂叶活性成分的提取纯化工艺条件。方法:以荭草素、异荭草素和金丝桃苷为指标,对5种树脂进行了筛选,并对选定的D101大孔树脂富集纯化荭草素、异荭草素及金丝桃苷的工艺条件进行考察。结果:D101型树脂对荭草、山楂叶中荭草素、异荭草素及金丝桃苷的吸附性能良好,在所确定的工艺条件下,荭草素、异荭草素和金丝桃苷转移率均大于91%,总固体物较树脂处理前减少60%以上。结论:D101大孔吸附树脂能有效富集纯化荭草、山楂叶中的活性成分。

Objective： To study the technical conditions of the extraction and purification of the active composition from Polygonum orientale and Crataegus pinnatifida Bge. in Hongye Xingtong soft capsules with the macroporous resin. Method： The orientm, isorientm and hyperoside were used as index to screen the five kinds of resins. And the technical conditions of the enrichment and purification of D101 resin selected out of above were all-round studied. Result： The D101 was fit for adsorbing orientm,isorientm and hyperoside. Under the optimal conditions, the transfer rate of orientm, isorientm and hyperoside was above 91% ,and the total solid was cut down by more than 60%. Conclusion： The D101 is greatly effective for the enrichment and purification of the active composition of P. orientale and C. pinnatifida Bge.