维脑康胶囊对慢性脑低灌注大鼠脑内胆碱能系统及清除自由基能力的影响

Effects of Weinaokang capsule on intracephalic cholinergic system and capability of scavenging free radicas in chronic cerebral hypoperfusion rats

目的：观察维脑康胶囊（WNK）对慢性脑低灌注所致大鼠认知障碍的干预作用及机制。方法：结扎大鼠双侧颈总动脉，4周后分为：假手术组，模型组，达纳康组，WNK8．25，16．5，33．0mg·kg^-1组；同时灌胃给药，给药周期8周，测定大鼠在MORRIS水迷宫中寻找平台的持续时间，同时HPLC—ECD测定脑组织中乙酰胆碱（ACh）含量，比色法测定乙酰胆碱酯酶（AChE）活性、超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量。结果：与模型组比较，给药4～8周，WNK组大鼠寻找平台时间明显缩短，脑内AChE活性明显降低（P〈0．05），ACh含量明显增加（P〈0．05～0．01），SOD活性明显增强（P〈0．05）。结论：WNK对慢性脑低灌注引起的认知障碍的改善作用可能与增强清除自由基能力以及保护胆碱能系统有关。

Objective： To observe the intervention and mechanism of Weinaokang capsule （WNK） on cognitive impairment rats induced by chronic cerebral hypoperfusion. Method： The bilateral common carotid arteries of rats were ligated. Rats were intragastrically administrated with WNK 4 weeks after the ligation. After 8 weeks of administration, persistent time of rats on finding the platform in MORRIS water maze was measured, meanwhile, content of acetylcholine （ACh） in brain tissue was measured by HPLCECD, activity of acetylcholine esterase （ACHE） and superoxide dismutase （ SOD）, content of malonaldehyde （MDA） was measured by colorimetric method. Result： After 4-8 weeks of administration, the time of finding the platform in WNK group was significantly shorter than in the model group, activity of ACHE in brain tissue was significantly reduced （P 〈 0. 05 ）, content of ACh was significantly increased （P 〈0. 05-0. 01 ）, activity of SOD was significantly reinforced （P 〈0. 05）. Conclusion： WNK capsule can improve the cognitive impairment induced by chronic cerebral hypoperfusion, and its mechanism may be associated with reinforcing the capability of scavenging free radical and protecting the cholinergic system.