钩藤散对AlCl_3所致老年痴呆模型鼠的影响

Effects of Goutengsan on model of alzheimer dementia in rats by AlCl_3

目的:观察钩藤散对老年性痴呆模型鼠脑指数,MAO-B,SOD,GSH-PX,NO,LDH等的影响,从而了解其作用机制。方法:120只大鼠随机均分为6组、实验组分高、中、低(5,2.5,1.25g·kg-1)3种剂量每天灌给钩藤散浸膏,模型组及正常组灌以等量生理盐水(0.01mL.g-1),阳性药组每天给予茴拉西坦胶囊(三乐喜,0.1g·kg-1),给药第5天时,除正常组外,各组腹腔注射AlCl3(100mg·kg-1),隔日1次,连续50d后,分别测定各组大鼠的死亡率、增重、学习成绩、脑指数、脑组织MAO-B,SOD,GSH-PX和血清NO,LDH等含量的变化。结果:实验组的脑指数显著(P<0.05)高于、SOD,GSH-PX活力和NO含量极显著(P<0.01)高于、LDH显著(P<0.05)低于、高、中剂量组的MAO-B含量极显著(P<0.01)低于、学习成绩和增重极显著(P<0.01)好于模型组,与正常组比,实验组除LDH偏高外其他各指标相近。结论:钩藤散能提高脑指数,降低死亡率,保持体重处于正常状况,增强抗氧化能力,抑制MAO-B活性,加强清除脂质过氧化物及其代谢废物的作用,减少LDH的漏出,维持NO于正常水平,从而保护细胞,抵抗痴呆。

Objective： Observe the effects of Goutengsan on SOD, MAO-B, GSH-PX, NO, LDH, index of brain, rate of death and so on in rats to study therapeutic effects and mechanism of Goutengsan on Alzheimer dementia （AD） model. Method： One hundred and twenty rats were randomly divided into 6 groups, 3 experimental groups of which were daily administrated with Goutengsan extract whereas the model and control groups were given NS （0.01 mL · g^-1 ）. Aniracetam at 0. 1 mL · g^-1 served as a positive control. At the 5th day after administration, all groups except the control were administrated （ip） with AlCl3 （ 100mL · g^-1 ） for successive 50 days at 1 day interval. After administration, the death rate, body weight, training scores, brain index, MAO-B, SOD, GSH- Px in brain and NO, LDH in serum were determined. Result： The brain index, SOD, GSH-Px activities as well as NO content of drugtreated groups were strikingly higher that of model group, and had not obvious difference from that of normal group except content of LDH was higher. Conclusion： Goutengsan could increase the brain index, cut down the rate of death, stable increase of body weight, promote the endogenous antioxidant activity, enhance the clearance of lipid peroxide and other metabolic waste. , inhibit the MAO-B activity, reduced the leakage of LDH and maintain the content of NO at a normal level. Therefore Goutengsan could protect cells, delay senile, improve symptoms of AD.