肝气郁证模型大鼠下丘脑单胺类神经递质分析

Analysis on the Monoamine Neurotransmitters in Hypothalamus of Rat Models of Liver-qi Stagnation Syndrome

目的探讨肝气郁证模型大鼠发病的微观机制。方法捆绑法造模制作肝气郁证模型大鼠,采用反相离子高效液相色谱(RP-IP-HPLC)测定大鼠造模后下丘脑中去甲肾上腺素(NE)、肾上腺素(E)、多巴胺(DA)及5-羟色胺(5-HT)4种神经递质含量变化。结果肝气郁证模型大鼠下丘脑中E、DA、5-HT水平显著升高(P<0.05或P<0.01),NE水平显著降低(P<0.01),且用药后均得到明显改善。结论肝气郁证与下丘脑中NE、E、DA、5-HT关系密切。

Objective To study the mechanism of liver-qi stagnation syndrome of rat models. Methods The binding method was used to make rat models of liver-qi stagnation syndrome and the quantitative changes of hypothalamus norepinephrine （NE）, epinephrine （E）, dopamine （DA）, and 5-hydroxytryptamine （5-HT） before and after modeling were determined with reversed phase ion pair （RP-IP）-HPLC. Results The level of E, DA and 5-HT was remarkably increased （P〈0. 05 or P〈0. 01） while NE decreased significantly （P〈0. 01）, and they were all improved after medication. Conclusion The liver-qi stagnation syndrome is closely related with level of hypothalamus NE, E, DA and 5-HT.