摘要
目的探讨肝气郁证模型大鼠发病的微观机制。方法捆绑法造模制作肝气郁证模型大鼠,采用反相离子高效液相色谱(RP-IP-HPLC)测定大鼠造模后下丘脑中去甲肾上腺素(NE)、肾上腺素(E)、多巴胺(DA)及5-羟色胺(5-HT)4种神经递质含量变化。结果肝气郁证模型大鼠下丘脑中E、DA、5-HT水平显著升高(P<0.05或P<0.01),NE水平显著降低(P<0.01),且用药后均得到明显改善。结论肝气郁证与下丘脑中NE、E、DA、5-HT关系密切。
Objective To study the mechanism of liver-qi stagnation syndrome of rat models. Methods The binding method was used to make rat models of liver-qi stagnation syndrome and the quantitative changes of hypothalamus norepinephrine (NE), epinephrine (E), dopamine (DA), and 5-hydroxytryptamine (5-HT) before and after modeling were determined with reversed phase ion pair (RP-IP)-HPLC. Results The level of E, DA and 5-HT was remarkably increased (P〈0. 05 or P〈0. 01) while NE decreased significantly (P〈0. 01), and they were all improved after medication. Conclusion The liver-qi stagnation syndrome is closely related with level of hypothalamus NE, E, DA and 5-HT.
出处
《中医杂志》
CSCD
北大核心
2008年第2期150-152,共3页
Journal of Traditional Chinese Medicine
基金
国家重点基础研究发展规划("973"计划)项目(2006CB504804)
关键词
肝气郁证
下丘脑
单胺类神经递质
高效液相色谱
Liver-qi stagnation syndrome
Hypothalamus
Monoamine neurotransmitters
High performance liquid chromatography (HPLC)