过氧化物酶体增殖物激活受体γ2基因Pro12Ala多态性与北京市汉族高血糖人群的胰岛素分泌相关

The PPARγ2 gene Pro12Ala polymorphism is associated with insulin secretion of hyperglycemia Han population in Beijing

目的研究过氧化物酶体增殖物激活受体(PPAR)γ2基因Pro12Ala多态性与北京市汉族人群肥胖、2型糖尿病的发生及胰岛素分泌的关系。方法使用口服75克葡萄糖耐量试验(OGTT)对北京市东城区非已知糖尿病汉族人群进行糖耐量检测,对其中的490例高血糖者(包括糖尿病及糖调节受损者)及585例糖耐量正常者进行病例对照研究。使用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RFLP)方法,对PPARγ2基因Pro12Ala多态性进行基因分型。结果病例组与对照组间PPARγ2基因Pro12Ala多态性频率分布无显著差别。病例组中,携带Ala12等位基因者的腰围明显增大(P=0.028);并且携带Ala12等位基因者的OGTT服糖后0.5,1及2小时的胰岛素水平明显低于对照组(P=0.026;P=0.040;P=0.038)。结论本研究提示在已有糖代谢紊乱的人群中,Ala12等位基因可能是加重其腹型肥胖并抑制其葡萄糖刺激后胰岛素分泌的一个因素。

Objective The purpose of this study is to investigate the association of Pro12Ala polymorphism at the PPARγ2 gene in Han population of Beijing with type 2 diabetes and islet function. Methotis Using oral 75g glucose tolerance test （OGTT） method,we systematically screen glucose tolerance in Han population in Dong-cheng district of Beijing. A case-control study comprised 490 hyperglycemia cases and 585 normal glucose tolerance controls. Using polymerase chain reaction based restriction fragment length polymorphism （PCR-RFLP） method,we genotyped Pro12Ala polymorphism in PPAR3,2 gene. Resuits There is no significant difference in PPAR3,2 gene Pro12Ala polymorphism genotype frequencies distribution between case and control. In case group, Ala12 allele was associated with larger waist （P = 0.028 ） and lower serum insulin after oral administration glucose 0.5,1,2 hour （P = 0. 026 ; P = 0.040 ; P =0.038 ;respectively）. Conclusions The results of our study suggest that Ala12 allele of PPAR3,2 gene could aggravate abdominal obesity and inhibit insulin secretion after glucose stimuli in hyperglycemia patients.