染色体核型及血清碱性磷酸酶水平在慢性粒细胞白血病病情预测中作用

The complex abnormal chromosome and the level of serum ALP in the prediction of the chronic myelocytic leukemia patients

目的研究慢性粒细胞白血病(CML)患者骨髓单个核细胞染色体核型与血清碱性磷酸酶(ALP)水平间变化关系,探讨细胞遗传学变化及ALP水平在患者病情及预后判断中的价值。方法选择不同时期CML患者,检测其血清ALP,并取骨髓有核细胞采用直接法或24小时培养法、R带显色技术作染色体分析。结果CML患者ALP水平高于正常对照组,加速期急变期患者复杂核型出现率及血清ALP水平显著高于慢性期患者,且ALP水平与髓内幼稚细胞数水平呈正相关,PH1阳性核型CML患者与复杂核型CML患者血清ALP水平差别不明显。结论CML患者血清ALP水平及染色体复杂核型反应该期患者疾病特征,对CML疾病分期、预后判断有价值,两者间的一致性有待进一步研究。

Objective To investigate and compare the level of serum alkaliphosphatase （ALP） and the chromosome in the patients with chronic myelocytic leukemia （CML）. Methods The serum concentration of ALP were indirectly tested by chemical method, Metaphase chromosomes were prepared using direct method and culture method. Karyotyping was performed using R-banding technique. All CML patients was not including those with immunologic disease.liver,kidney disease and infective disease and were divided into the chronic phase CML and the acceleration phase or blast crisis CML. The normal group was 20 cases. Results Two group patients were higher level of ALP than the normal group. The acceleration phase or blast crisis CML patients has not only the high incidence of the complex abnormal chromosome but also the high levels of ALP than the chronic phase patients, the serum ALP level have the positive correlation with infantility cells too, but the difference of the level of serum ALP was not found between those with PH1 and those with the complex abnormal chromosome. Conclusions The high serum ALP level and high incidence of the complex abnormal chromosome in acceleration phase or blast crisis CML patients closely related their situation.