流动注射抑制化学发光法测定盐酸多西环素和土霉素

Flow injection inhibitory chemiluminescence for the determination of doxycycline and oxytetracycline hydrochloride

在酸性条件下,Ce（Ⅳ）-罗丹明6G发光体系有较强的化学发光,盐酸多西环素及土霉素的存在能抑制其发光。且化学发光信号的减小值（ΔI）与盐酸多西环素及土霉素的浓度在一定的范围内有较好的线性关系。据此结合流动注射技术,建立了快速测定盐酸多西环素及土霉素的流动注射化学发光分析新方法。优化了影响化学发光的各种因素。结果表明在优化的条件下,盐酸多西环素及土霉素的线性范围分别是1.0×10^-7-1.0×10^-4g/mL,1.0×10^-6-1.0×10^-4g/mL,测定的检出限为5.0×10^-8g/mL及2.7×10^-7g/mL,对1.0×10^-6g/mL的盐酸多西环素及1.0×10^-5g/mL的盐酸土霉素分别进行了9次平行测定,其相对标准偏差分别为1.4%、1.6%。方法已用于盐酸多西环素及土霉素片剂的测定。

In acid solution, Ce（ Ⅳ）-Rhodamine 6G system has a great chemiluminescence, the chemilumimescence intensity was inhibited in the presence of doxycycline and oxytetracycline hydrochloride. The inhibitory intensity was linear with the concentrations of doxycycline and oxytetracycline hydrochloride. Coupled with the flow injection technique, a rapid flow injection chemiluminescence method was developed for the detennination of doxycyline and oxytetracycline hydrochloride. The parameters which affects the chemiluminescence signal were investigated. Under the optimized conditions, the linear range of 1.0 × 10^-7 - 1.0 × 10^-4 g/mL, 1.0 × 10^-6 - 1.0 × 10^-4 g/mL, with the detection limit of 5.0 × 10^-8 g/mL, 2.7 × 10^-7 g/mL were obtained for doxycychne hydrochloride and oxytetracycline hydrochloride, respectively. The RSDs of 1.4% and 1.6% were obtained for nine times consecutive determination of 1.0 × 10^-6 g/mL doxycycline hydrochloride and 1.0 × 10^-5 g/mL oxytetracycline hydrochloride, respectively. Satisfactory results were obtained with the method applied for the pharmaceutical preparations of doxycycline and oxycycline hydrochloride.