动力学荧光法测定对苯二酚的研究

A study on kinetic fluorimetric determination of hydroquinone

基于在稀H2SO4介质中,对苯二酚对H2O2氧化罗丹明B产生的荧光猝灭有显著的催化作用,建立了测定对苯二酚的动力学荧光分析法。反应在80℃的水浴中进行12 min,方法的线性范围为4.0-600.0μg/L,检出限为3.7μg/L。该方法用于水样中对苯二酚的测定,加标回收率在96.4%-101.3%之间,相对标准偏差为2.2%。

A new kinetic fluorimetric method was proposed for the determination of trace hydroquinone. The method is based on the catalytic effect of trace hydroquinone on the oxidation of rhodamine B by hydrogen peroxide in sulfuric acid medium. The reaction runs in 80 ℃ water for 12 min. The linear range of the determination is 4.0 - 600.0μg/L and the detection limit is 3.7 μg/L. The method has been applied to determining hydroquinone in water samples. And the recoveries between 96.4% and 101.3% are obtained. The RSD is 2.2%.