摘要
为全面了解N-ras癌基因突变与急性髓细胞白血病(AML)亚型的关系,从新鲜骨髓和库存骨髓片提取DNA,应用银染聚合酶链反应-单链构型多态性(PCR-SSCP)技术对98例AML患者的N-ras癌基因第1和第2外显子的点突变进行了研究。结果显示:共有29例检测到突变,其中第1和第2外显子突变分别为18例和14例,两外显子同时突变3例;突变率为29.59%。除M[7]未检测外,其它各亚型均发现突变;而以M4a为多见,M[3a]为少见。因此,N-ras癌基因突变是AML常见的肿瘤标志。
In order to understand the relationship of N-ras oncogene mutation and the subtype of acute myeloid leukemia (AML),the DNA was extracted from fresh bone marrow and stored bone marrow slides,then the point mutation in exon Ⅰ and exon Ⅱ of N-ras oncogene in 98 patients with AML were studied by polymerase chain reactionsingle strand conformation polymorphisms (PCR-SSCP) and silver staining method.The results demonstrated that the point mutations had been detected in 29 cases (18 cases of exon Ⅰ ,14 cases of exon Ⅱ ,3 cases of both exon Ⅰ and Ⅱ).The total mutation rate was 29.59%.All subtypes but M 7 were found mutations,of which M 4a were commonly found and M 3a rarely found;and this indicates that the point mutation of N-ras oncogene is a commonly tumour marker in AML.
出处
《赣南医学院学报》
1997年第3期197-200,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
江西省自然科学基金
关键词
白血病
髓细胞性
急性
PCR-SSCP
N-ras癌基因
Leukemia,myelocytic,acute/analysis
polymerase chain reactionsingle strand conformation polymorphisms
N-ras oncogene